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SAFINURTM
(Cancer Research) |
Safinur
completely stops cell
growth/proliferation,
cell movement and cell
invasion. Because of
this multiplicity of
effects, we
investigated whether
Safinur was affecting
a general cellular
mechanism. The Safinur
molecule has two
active ends. One end
helps the molecule
enter the cancer cell
through membrane
transporters. Cancer
cells upregulate these
transporters allowing
for the increased
uptake of Safinur in a
selective fashion.
Once inside, the other
end of the Safinur
molecule irreversibly
disrupts the
cytoskeleton. |
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The
cytoskeleton is
a non-redundant
biological
structure, which
is essential for
cell survival.
Cellular growth,
morphology,
mobility,
invasiveness and
nutrition are
processes that
are dependent on
the cytoskeleton,
which is common
to all cells. It
is an integral
part of the cell
contributing to
all its
physiological
activity. To
illustrate
Safinur's
disruption of
the cytoskeleton,
adherent breast
carcinoma cells
(MCF-7) grown on
chamber slides
were treated
with Safinur for
2 h, fixed, and
stained with a
cytoskeleton
specific primary
monoclonal
antibody. As
shown in the
accompanying
figure in panel
A, Safinur
treatment
dramatically
disrupts the
organization of
cytoskleleton
filaments within
the cells. This
disruption
coincided with
the 2-h time
frame for cell
disruption
observed
previously.
Panel B
demonstrates
that Safinur
directly
disrupted
cytoskeletal
filaments in a
cell-free
assay. The
nature and
kinetics of the
disruption is
currently under
investigation. |
(A)
Safinur
disrupts
cytoskeleton
filaments.
Immunofluorescence.
Adherent
MCF-7
cells
were
incubated
for
2
h
±
0.01%
Safinur.
Cytoskeleton
filaments
were
stained
with
a
primary
antibody
and
TRITC-anti-mouse
IgG.
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Control |
Safinur-treated |
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(B)
Purified
cytoskeleton.
cytoskeleton
monomers
(pre-incubated
for
1
h
±
0.01%
Safinur)
was
polymerized
on
a
glass
slide.
Filaments
were
visualized
with
TRITC-phalloidin.
Magnification:200X
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Control |
Safinur-treated |
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Educational
corner:
Examples
of
interrupted
cell
activities
as
a
result
of
cytoskeleton
disruption |
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Why
is disrupting
the cytoskeleton
important in
combating cancer
cells? Cancer
cells are highly
mitotic, highly
voracious and
highly mobile
and because all
these activities
are cytoskeleton
dependent, its
disruption is a
major anti-tumor
mechanism. |
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Example
1:
The
process of
cytokinesis
(division of
cells) is
dependent on the
cytoskeleton. In
the picture
below, two cells
in the process
of dividing,
bud-off with the
help of a
cytoskeletal
contractile
ring. This
process is
prevented from
occurring in the
presence of
Safinur. |
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2:
One
process by which
cells uptake
nutrients is
that of
phagocytosis and
endocytosis.
These processes
are dependent on
the
reorganization
of the
cytoskeleton. By
disrupting the
cytoskleton,
Safinur prevents
the cell from
uptaking
nutrients. |
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3:
Cellular
mobility is
dependent on the
reorganization
of the
cytoskeleton.
Unlike our bone
skeleton, which
is relatively
stable, the
cytoskeleton is
more dynamic,
reorganizing in
a relatively
fast process.
This process
occurs by the
tread milling of
protein monomers
into larger
filaments
necessary for
cellular
mobility.
Safinur disrupts
this tread
milling process
by chelating the
monomers and
preventing their
uptake by the
growing
filaments
(below). |
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If
you have any questions
regarding information
contained in this
website, please give
us a call at
714-532-4632 or email
us at info@safinur.com |
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Saficor,
Orange, California USA; Copyright Saficor
2006 all rights reserved
Disclaimer
The information contained here is
not intended as a substitute for medical professional help or advice but is to
be used only for informational purposes. A physician should always be consulted
for any health problem or medical condition. Safinur has not yet undergone
organized clinical trials for cancer. It is provided as a dietary supplement for
clinical study. Before taking this or other dietary supplements consult your
physician. Safinur has been shown to be uniquely non-toxic in many animal
studies; however, systematic human studies on toxicity have not been conducted.
Statements made on this website have not been evaluated by the FDA and are not
meant to diagnose, treat, cure or prevent any disease.
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